The hyphal form and biofilm formation in C. albicans is widespread, with an increasing prevalence of 18–25% in the last few decades. Surgery (especially abdominal surgery), burns, long-term hospitalization in an intensive care unit, and earlier use of broad-spectrum antibiotics and immunosuppressive drugs have all been risk factors for invasive candidiasis. albicans is of significant clinical importance as it is responsible for causing superficial to invasive candidiasis. In healthcare institutions, candidiasis is still the most frequent hospital-acquired fungal infection, and almost 0.25 million people suffer from invasive candidiasis every year. Fungal infections spread drastically over the past few decades, and annual fatalities from fungal infections are higher than individually from TB, HIV, malaria, or breast cancer. Commensal mycobiota can act as pathobiont in compromised host immunity and under certain clinical conditions. Mycobiota, like other microbiota, is an essential part of the human body and resides in the genitourinary tract, gastrointestinal tract, respiratory tract, skin, and the mucosal membrane covering the oral cavity. In cirrhosis and renal impairment, absorption and plasma accumulation increased so we recommend further investigation into this occurrence and recommend high dosage in further tests to increase bioavailability. Pharmacological based pharmacokinetic modeling revealed that it has low bioavailability and hence, absorption in healthy state. (4-Hydroxybenzyl)thiocarbamic acid showed hepatotoxicity, while Chelerythrine depicted hERG inhibition, which can lead to QT syndrome, so we recommend ZINC13507461 for further testing in lab. No Ames toxicity was predicted for prioritized compounds while control depicted this toxicity. The top compound selected on the basis of best docking score from traditional Indian medicine/Ayurvedic library was (4-Hydroxybenzyl)thiocarbamic acid, from the ZINC FBA inhibitor library was ZINC13507461 (IUPAC name: (9E,12E)-octadeca-9,12-dienoate), and from traditional Tibetan medicine/Sowa rigpa was Chelerythrine (IUPAC name: 1,2-Dimethoxy-12-methyl-9H-benzodioxolophenanthridin-12-ium), compared to the control (2E)-1-(4-nitrophenyl)-2-hydrazine. In this study, we attempted to mine drug targets ( n = 46) using a subtractive proteomic approach in this pathogenic yeast and screen natural products with inhibition potential against fructose-bisphosphate aldolase (FBA) of the C. Excess of Candida albicans causes candidiasis, causative of thrush and vaginal infection due to off-balance. Despite being responsible for invasive infections, fungal pathogens have been underrepresented in computer aided therapeutic target mining and drug design.
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